Big ET
Produktdaten
Big Endothelin
Précautions
Centrifuge freshly collected blood as soon as possible
Store centrifuged samples at -20°C for longer storage.
Samples are stable up to 4 freeze and thaw cycles.
Hemolyzed or lipemic samples may cause erroneous results.
Valeurs de référence
Median (serum) = 0.09 pmol/l (n = 41)
| Nom du produit | Big ET |
| Cat. no | BI-20082H |
| Gamme | 0 – 3 pmol/l |
| Sensibilité | 0.03 pmol/l (STD2 0.1 pmol/l) |
| Durée d'incubation | 3.5 hours |
| Volume échantillon | 50 µl |
| Echantillon | Serum, plasma |
| Espèces | Human |
| Tests | 96 |
| Méthode | ELISA |
Intérêt clinique
Intended use:
Big Endothelin-1 (BigET) is a peptide of 38 amino acids and is the precursor of Endothelin-1 (ET), represented by amino acids 1-21 (http://www.uniprot.org/uniprot/P05305). ET is a potent vasoconstrictor and is produced by vascular endothelial cells. Accordingly it has a wide tissue distribution (http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.511899). The cleavage of BigET by Endothelin Converting Enzyme (ECE) leads to ET and to a C-terminal fragment. Both BigET and ET are strong independent predictors of survival in patients with congestive heart failure, and identify a population with very high risk mortality. The half-life of ET (1-21) in plasma is less than one minute, whereas clearance of BigET is much slower. BigET can therefore be determined more easily.
Intended applications:
- atherosclerosis
- prognostic value in heart failure and acute myocardial infarction
- renal insufficiency during and after graft rejection
- pulmonary hypertension and scleroderma