Complément Anticorps Monoclonaux

paraffin embedded tissue sections, frozen sections

Human

The antibody has been tested for use on paraffin sections and frozen sections of human tissue. Working protocol available. Recommended dilution for immunohistochemistry: 1:30 – 1:50 Pretreatment of slides by pressure cooking (5 minutes at +125°C in TRIS/EDTA buffer pH 8.5) is highly recommended for antigen retrieval.

Intended use:

C4d is the degradation product of the activated complement factor C4, a component of the classical complement cascade, which is typically initiated by binding of antibodies to specific target molecules. Detection of C4d is regarded as an indirect sign, a "footprint" of an antibody response against the allograft. The majority of publications describe C4d as an important biomarker in kidney transplantation but also in heart, liver, and other transplants.

Intended applications:

• Humoral transplant rejection