Centrifuge freshly collected blood as soon as possible
Store centrifuged samples at -20°C for longer storage.
Samples are stable up to 4 freeze and thaw cycles.
Hemolyzed or lipemic samples may cause erroneous results.
Median (serum) = 0.09 pmol/l (n = 41)
|Product name||Big ET|
|Range||0 – 3 pmol/l|
|Sensitivity||0.03 pmol/l (STD2 0.1 pmol/l)|
|Incubation time||3.5 hours|
|Sample volume||50 µl|
Big Endothelin-1 (BigET) is a peptide of 38 amino acids and is the precursor of Endothelin-1 (ET), represented by amino acids 1-21 (http://www.uniprot.org/uniprot/P05305). ET is a potent vasoconstrictor and is produced by vascular endothelial cells. Accordingly it has a wide tissue distribution (http://www.ncbi.nlm.nih.gov/UniGene/ESTProfileViewer.cgi?uglist=Hs.511899). The cleavage of BigET by Endothelin Converting Enzyme (ECE) leads to ET and to a C-terminal fragment. Both BigET and ET are strong independent predictors of survival in patients with congestive heart failure, and identify a population with very high risk mortality. The half-life of ET (1-21) in plasma is less than one minute, whereas clearance of BigET is much slower. BigET can therefore be determined more easily.
- prognostic value in heart failure and acute myocardial infarction
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- pulmonary hypertension and scleroderma