Apoptosis
Product name | M65® ELISA (PEVIVA®)Apoptosis + Necrosis. ![]() |
Cat-Nr. | 10020 |
Range | 125 - 2000 U/L (The Units measured by the M65® ELISA are defined against a synthetic standard. 1 U/L = 1.24 pM.) |
Sensitivity | 25 U/L |
Incubation time | 2 hours 20 minutes |
Sample volume | 25 µl |
Sample type | serum, plasma (EDTA, Heparin, Citrate) and cell (epithelial cell |
Sample preparation | Store samples at 2 – 8 °C up to 4 hours. For longer |
Reference values | Median level 264 U/L with a range between 136 – 480 U/L. |
Species | Human , primates, bovine |
Specificity | The assay uses two monoclonal antibodies directed to epitopes in the |
Tests | 96 |
Method | ELISA |
Wiederfindung | The Standard provided with the kit contains recombinant material which is not comparable to the fragment measured in the sample and is not adequate for spiking recovery tests. The recovery for the recombinant K18 protein fragment observed in human serum/plasma will be higher than expected. |
Verduennung | Recovery for human sera when diluted 1:2 in M65 Standard A (0 Units/L): 126 % (average) and 116 – 139 % (range). |
Hook | No high dose “hook effect” occurs before 70 000 U/L. |
Intended use | Quantitative measurement of total soluble cytokeratin 18 (K18) released from dead cells (necrotic and apoptotic). The cells or tissues should be of human epithelial origin (e.g. kidney, gut, colon, lung or liver) expressing K18. The M65® ELISA can be combined with the M30-Apoptosense® ELISA (PEVIVA Prod. No. 10010) for determination of cell death mode (apoptosis versus necrosis). Death mode can be determined in vitro and in serum from cancer patients (Kramer et al., Cancer Res. 2004). |
Product informations |