M65 EpiDeath® ELISA (PEVIVA®)
M65 Apoptosis + Necrosis. Human intermediate filament protein keratin 18 (K18)*. Released from human epithelial cells.
Store the samples at 2–8 °C up to 4 hours. For longer periods, store samples at -20 °C or lower.
Avoid repeated freezing and thawing of specimens.
|≤ 128 U/l||Normal|
|128 – 183 U/l||Greyzone|
|> 183 U/l||Elevated (if carcinoma is excluded)|
|Product name||M65 EpiDeath® ELISA (PEVIVA®)|
|Range||200 - 5000 U/l ( 1 U/l = 1.24 pM)|
|Incubation time||4.5 hours|
|Sample volume||25 µl|
serum, plasma (EDTA, Heparin, Citrate) and cell cultures (epithelial cell)
The assay uses two monoclonal antibodies directed to epitopes in the 284 – 396 region of the K18 protein. Soluble full length K18 as well as K18 fragments and protein complexes that expose these epitopes will be detected by the assay.
No high dose “hook effect” occur until 200000 U/l which is well above concentrations of M65-reactive material observed in cell culture or serum/plasma samples.
Quantitative measurement of total soluble keratin 18 (K18) released from dead cells (necrotic and apoptotic). The cells or tissues should be of human epithelial origin (e.g. kidney, gut, colon, lung or liver) expressing K18.
The M65 EpiDeath® ELISA can be combined with the M30 Apoptosense® ELISA (PEVIVA Prod. No. 10010) for determination of cell death mode (apoptosis versus necrosis). Death mode can be determined in vitro and in serum from cancer patients.
caspase-cleaved K18 = ccK18 previously Cytokeratin 18 (CK18/ccCK18)