Centrifuge freshly collected blood as soon as possible
Store centrifuged samples at -20°C for longer storage.
Samples are stable up to 3 freeze and thaw cycles.
Hemolyzed or lipemic samples may cause erroneous results.
Median = 34 pmol/l (n = 51)
|Range||0 – 160 pmol/l|
|Sensitivity||1.25 pmol/l (STD2 10 pmol/l)|
|Incubation time||3.5 hours|
|Sample volume||20 µl|
Dickkopf-1(DKK-1) is a 28.67 kDa secreted protein that acts as soluble inhibitor of the WNT signalling pathway. This pathway contains lipid-modified glycoproteins that activate cell surface receptor-mediated signal transduction to regulate cell activities like: cell fate, proliferation, migration, polarity and gene expression.
DKK-1 regulates developmental processes of all kinds. Thus, DKK-1 is also involved in the regulation of bone metabolism as it effects osteoblast differentiation and in regulation of tumourgenetic activity.
- Rheumatoid arthritis
- Myeloma bone disease
- Diffuse idiopathic skeletal hyperostosis
- Ankylosing spondylitis
- Monoclonal gammopathy of undetermined significance
- Glucocorticoid induced osteoporosis