Cartilage metabolism

Product name

Hyaluronic Acid - Hyaluronan (HA) ELISA

Range15 – 1000 ng/mL (Pharmacopeia Standard)
LOD: . 2.7 ng/ml
LLOQ: 15 ng/ml
ULOQ: 1000 ng/ml
Sensitivity10 ng/ml
Incubation time3 hours
Sample volume100 µl
Sample type

Serum, EDTA-plasma and cell culture supernatant

Sample preparation

Fasting blood collection. Serum or EDTA Plasma stable for 72 hours at 2-8 °C, 6 months at -20°C, longer storage at -80°C.
Maximum 3 freeze- and thaw cycles.

Reference values

Hyaluronic Acid Values are dependent on age and gender and influenced by food intake and physical activity.

Clinically Healthy Subjects n=53 between 16 and 79 years.
36.7 ± 23.5 ng/ml.

  Mean (ng/ml) SD (ng/ml)
Female premenopausal 20,1 14,3
Female postmenopausal 50,3 19,9
Male 42,6 24,6

 Cutoff 90 ng/ml ± 2 SD



Tests96 Tests
Intended use

Diagnosis and monitoring of liver fibrosis and cirrhosis
Diagnosis and monitoring of joint diseases
HA is also important in diabetes, as a tumor marker and
in pulmonary infections.

Product informations

Hyaluronic acid (HA), also known as hyaluronan or hyaluronate is synthesized by fibroblasts and other specialized connective tissue cells and is a major component of connective tissues and thus distributed ubiquitously in the organism. HA can act as a biomarker for joint diseases and correlates in RA and OA patients with the degree of joint inflammation, synovial proliferation and the degree of joint space narrowing. Due to the decreased capacity for hyaluronan clearance, HA is an important biomarker for most chronic liver diseases which are characterised by fibrosis and cirrhosis. Our Hyaluronic Acid PLUS ELISA is suited for the determination of hyaluronic acid in plasma, serum and other biological fluids.

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