Collagen resorption. C-Terminus of 3/4 peptide, generated through cleavage of types I and II collagens by collagenases
Serum samples should be aliquoted, rapidly frozen and stored at -70 °C. Avoid repeated freezing/thawing of samples.
Serum: stable > 1 year at -70 °C.
|Range||0.03 - 10 µg/ml|
|Incubation time||2.5 hours|
|Sample volume||25 μl (dilute serum 1:2)|
Serum (also used for plasma, urine, cell culture, tissue, synovial fluid, cartilage extracts, bronchoalveolar lavage)
Human (also used for dog, bovine, horse, mouse, rhesus macaque, rat, guinea pig, sheep). The use of rabbit samples may lead to high background interference.
Negligible cross-reaction with uncleaved triple-helical and heat-denatured human collagen types I and II and intact or cleaved alpha-chains of collagen type-II.
C1,2C as a biomarker is used in the investigation of the progression of rheumatoid arthritis, osteoarthritis, ankylosing spondylitis, reflecting cartilage, ligament, skin, synovium/capsule, lung and bone damage or inflammation or repair, in human as well as some animal models. Increased serum C1,2C concentrations give an indication of a rapid progressive joint damage in RA.