Apoptosis

Product name

M65 EpiDeath® ELISA (PEVIVA®)

67 – 5000 U/l (The units measured by the M65 EpiDeath® ELISA are defined against native antigen spiked into serum. Native antigen is calibrated against a recombinant protein standard.1 U/l = 1 .24 pM).

Cat-Nr.10040
Range200 - 5000 U/l ( 1 U/l = 1.24 pM)
Sensitivity25 U/l
Incubation time4.5 hours
Sample volume25 µl
Sample type

serum, plasma (EDTA, Heparin, Citrate) and cell cultures (epithelial cell)

Sample preparation

Store samples at 2 – 8 °C up to 4 hours. For longer periods, store samples frozen at -20 °C or lower.
Samples can be freeze-thawed without loss of activity, it is recommended that repeated freezethawing should be avoided.

Reference values
   
   
   

200 normal subjects, 95th percentile 266 U/L
Total soluble K18 (uncleaved and cleaved Keratin 18 representing total cell death (necrosis and apoptosis) was measured in 200 apparently normal Swedish blood donors. Male and female values showed a similar distribution and no age dependency. Based on the distribution the recommended cut-off value for elevated K18 has been set at > 200 U/L.

Species

Human, non-human primates

Specificity

The assay uses two monoclonal antibodies directed to epitopes in the
284 – 396 region of the K18 protein. Soluble full length K18 as well as K18 fragments and protein complexes that expose these epitopes will be detected by the assay.

Tests96
MethodELISA
Hook

No high dose “hook effect” occur until 200000 U/l which is well above concentrations of M65-reactive material observed in cell culture or serum/plasma samples.

Intended use

Quantitative measurement of total soluble keratin 18 (K18) released from dead cells (necrotic and apoptotic). The cells or tissues should be of human epithelial origin (e.g. kidney, gut, colon, lung or liver) expressing K18.

The M65 EpiDeath® ELISA can be combined with the M30 Apoptosense® ELISA (PEVIVA Prod. No. 10010) for determination of cell death mode (apoptosis versus necrosis). Death mode can be determined in vitro and in serum from cancer patients.


*Note:
caspase-cleaved K18 = ccK18 previously Cytokeratin 18 (CK18/ccCK18)

Product informations

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