Dosages de l’activité du complément par méthode ELISA

Nom du produit

C3a Mouse (TECO®)

Cat. noTE1038
Gamme31 – 500 ng/ml
SensibilitéLOD: 4.5 ng/ml LLOQ: 0.023 ng/ml
Durée d'incubation3 hours
Volume échantillon25 μl (plasma ou sérum, dilué 1:100)
Echantillon

Mouse plasma, serum or other biological fluids.

Précautions

Non-lipemic samples are recommended. Centrifuge collected blood samples within 4 hours. Use immediately in the Assay or keep frozen at - 80°C. Repeated freeze and thaw cycles should to be avoided.

Valeurs de référence

5 – 20 µg/ml

Espèces

Mouse

Tests96
MéthodeELISA
Intérêt clinique

C3 is central to the classical, alternative and lectin pathways of complement activation.

The synthesis of C3 is tissue‐specific and is modulated in response to a variety of stimulatory agents. During complement activation, C3 is proteolytically cleaved resulting in release of the anaphylatoxic peptide C3a.

 

C3a is a small polypeptide consisting of 74 amino acids. C3a itself is very short‐lived and in serum cleaved rapidly into the more stable C3a‐desArg (also called acylation stimulating protein, ASP). Therefore, measurement of C3a‐desArg allows reliable conclusions about the level of complement activation in the samples.

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