Oxidative Stress & Cardiovascular Disease

Product name

Big Endothelin

Cat-Nr.

BI-20082H

Range

0 – 3 pmol/l

Sensitivity

0.10 pmol/l

Incubation time

5.5 hours

Sample volume

50 µl

Sample type

Serum, plasma

Sample preparation

Centrifuge freshly collected blood within one day.
Samples should be stored at -20°C, for long time storage store at -70°C. Maximum 4 freeze-thaw cycles.

Reference values

Serum: Median: 0.09 pmol/l (n= 41)

Species

Human, porcine & rat

Cross reaction

ET1/2/3 (1-21)	<1%
ET2 (1-37)	<1%
ET1/2 (1-38)	<1%
pig Big ET (1-39)	21%
Big ET-1/2 (22-38)	<1%
Big ET-2 (22-37)	<1%
Sarafotoxin	<1%
rat Big ET-1 (1-39)	10%

Tests

Method

ELISA

Intended use

Big Endothelin-1 (Big ET), a small 38-amino-acid peptide, is a biological precursor of Endothelin (1-21), the most potent vasoconstrictor known today. Various cell types including vascular endothelial cells and non-vascular cells (eg. Mesangial, kidney and epithelial cells) produce Endothelin.
Cleavage of Big Endothelin by a membrane-bound metalloproteinase, the Endothelin Converting Enzyme, leads to the active Endothelin (1-21) and to the biological inactive C-terminal fragment (22-38).
The physiological importance of cleavage of Big Endothelin is indicated by the reported 140-fold increase in vasoconstrictor activity upon cleavage to Endothelin-1, although both peptides can be determined in about equimolar concentrations in plasma.
Both Big ET and ET are strong independent predictors of survival in patients with congestive heart
failure, and identify a population with a very high risk mortality.
It was demonstrated that the half-life of Endothelin (1-21) in the plasma is less than one minute, whereas clearance of Big Endothelin is is much slower. BigET can therefore be determined more easily.

Clinical significance:

prognostic value in heart failure and acute myocardial infarction
renal insufficiency
during an after graft rejection
atherosclerosis
pulmonary hypertension
scleroderma

Product informations

Kit Instructions (PDF 433,2 kB)

Cross-reaction all species (PDF 74,6 kB)

The Cardiovascular Product Line (PDF 2,0 MB)

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