Oxidative Stress & Cardiovascular Disease

Product name

BNP Fragment

Cat-Nr.BI-20852W
Range0 – 6400 pmol/l
Sensitivity200 pmol/l
Incubation timeover night at 4°C / 20 min RT
Sample volume30 µl
Sample type

Serum, plasma

Sample preparation

Centrifuge freshly collected blood as soon as possible.
Samples should be stored at -20°C, for long time storage at -70°C.
Max. 5 freeze-thaw cycles.
Lipemic or hemolyzed samples may give erroneous results.

Reference values

blood donors (n=76): median = 392 pmol/l

Species

Human

Tests96
MethodELISA
Intended use

 

The natriuretic peptides are members of a family of structurally similar but genetically distinct
peptide hormones, consisting of atrial-, brain-, and C-type (ANP, BNP, and CNP, respectively). ANP
and BNP preferentially bind to a membrane-bound guanylyl cyclase (GC) receptor called GC-A or NPR1,
whereas CNP is the physiological ligand for GC-B (NPR2).
The natriuretic peptides play an important role in the regulation of cardiovascular and renal homeostasis and in the regulation of fatty acid metabolism and body weight.
BNP is mainly expressed by ventricular myocardium in response to volume overload and increased
filling pressure. BNP has a cleavable signal sequence. Mature BNP consists of 108 amino acids (proBNP or BNP-108), and undergoes cleavage resulting in physiologically active BNP-32 and additional C-terminal fragments along with a physiologically inactive N-terminal peptide comprising amino acids 1-76, which is further degraded proteolytically. BNP fragments in the circulation are therefore very heterogenous.
BNP has a key role in cardiovascular homeostasis with biological actions including natriuresis,
diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. A high concentration
of BNP in the bloodstream is indicative of heart failure.
The discovery of natriuretic peptides identified an endocrine system that contributes to diuresis
and vascular tone.

Clinical significance:

  • Cardiac impairment
  • acute myocardial infarction (left ventricular dysfunction)
  • renal failure
  • obesity and diabetes
  • various forms of secondary hypertensions

 

Product informations

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