Growth metabolism

Product name

IGFBP-3 Mouse / Rat

Insulin like Growth Factor Binding Protein- 3 for mouse and rat

Cat-Nr.E031
Range23.5 - 1505 µg/l (after dilution 1:301)
Sensitivity0.018 ng/ml
Incubation time2.5 hours RT
Sample volume100 µl (pre-diluted 1:301)
Sample type

Serum, cell lysates, nuclear extracts.

Sample preparation

Samples have to be chilled as soon as possible after collection. Long-term storage should be carried out at –20°C or below. Maximum 5 freeze/thaw cycles.

Reference values
Mouse 200 - 800 ng/ml
Rat 200 - 500 ng/ml

 

Species

Mouse, rat

Tests96 Tests
MethodELISA
Intended use

Growth Hormone, Insulin-like Growth Factors and their binding proteins build up an endocrine system regulating not only longitudinal growth in humans but also influencing a broad variety of other physiological and pathophysiological processes like energy metabolism or tumor growth. Most effects of Growth Hormone (GH) are exerted by Insulin-like Growth Factors (IGF) mainly produced by liver but also locally by specific tissues. The effects of IGF are regulated, specific binding proteins (IGFBP 1-7) regulate bioavailability of IGF. After proteolytic cleavage of the binding proteins IGF is set free and able to bind to its receptor. The autophosphorylation of this thyrosine kinase receptor activates intra cellular signaling cascades. Some of these IGFBPs not only regulate the availability of IGF but also exert IGF-dependent effects on cell physiology.

IGFBP-3 is the most abundant IGFBP in circulation and therefore of special relevance in regulation of IGF effects. This is reflected by the indicative value of serum IGFBP-3 concentration in diagnostics of growth disturbances. IGFBP-3 has also been shown to be able to induce apoptosis, promote tumor growth and inhibit cellular migration and metastasis dependent on tissue and tumor stage.

Product informations

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