Growth metabolism

Product name

IGFBP-3

Insulin like Growth Factor Binding Protein- 3

Cat-Nr.E03A
Range0.4 - 30 ng/ml
Sensitivity0.1 ng/ml
Incubation time2.5 hours RT
Sample volume10 ┬Ál (dilute 1:505 for serum and plasma)
Sample type

Serum, plasma (heparin), CSF, cell culture. Results in citrate or EDTA plasma are about 15% reduced.

Sample preparation

Samples have to be chilled as soon as possible after collection. Long-term storage should be carried out at –20°C or below. Maximum 5 freeze/thaw cycles.

Reference values

In contrary to GH IGFBP-3 shows stable serum levels due to absence of circadian variation. In children IGFBP-3 levels are strongly age and gender dependent. A detailed table is included in the product brochure.

Species

Human

Tests96 Tests
MethodELISA
Intended use

IGFBP-3 is a peptide of 264 amino acids (MW 29kDa), forming a complex with a 150 kDa glycoprotein (ALS, acid labile subunit) and one molecule of IGF. In serum, approximately 95% of IGF-I and IGF-II are bound to IGFBP-3, making this protein to the main carrier of the circulating insulin-like growth factor. The function of the binding protein is to prolong the half-life of the circulating growth factor towards several hours. The determination of IGFBP-3 has been proposed as the best indicator of GHD during the five first years of life because of the low discrimination value of IGF-1 levels. A single IGFBP-3 determination is an excellent screening parameter for GHD and IGFBP-3 is a good parameter for monitoring the therapeutic efficacy in both GHD and acromegaly.

Product informations

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