Apoptosis

Product name

M30 Apoptosense® ELISA (PEVIVA®)

Measurement of soluble human intermediate filament protein fragments of keratin 18 (K18)* that contain the M30 neo-epitope (K18Asp396-NE). Released from human epithelial cells.

Cat-Nr.10011
Range75 – 1000 U/l (units are defined against a synthetic peptide standard containing the M30 and M5 epitopes; 1 U/l = 1.24 pM)
Sensitivity25 U/l
Incubation time4.5 hours
Sample volume25 µl
Sample type

Human serum or plasma (EDTA, citrate, heparin plasma), K18Asp396-NE (M30)-reactive material released from apoptotic K18 positive human cells. The same type of material, i.e. serum or plasma collected by one method, should be used for a specific project .
Mouse plasma can be used for measurement of human xenografts. Reference: Olofsson et al, Cancer Biomark , 2009 
Cell lysates or cell culture supernatants from K18 positive (epithelial) apoptotic cells or tissues – For cell culture samples, the M30 Apoptosense®  ELISA or the M30 CytoDeath™ ELISA can be used.
 

Sample preparation

Blood samples are stable for 48 hours at room temperature*
Samples (serum or plasma) can be stored for up to 7 days at room
temperature, 2 weeks at 2-8°C, longer term sample storage at minimum -
20°C.
Repeated freeze-thawing procedures do not affect the reactivity of the
samples.

Reference values

Healthy                       ≤ 150 U/L
Slightly elevated       150 – 200 U/l
Elevated                     > 200 U/l

CcK18 values were measured in 200 apparently normal Swedish blood donors. With the M30 Apoptosense® ELISA, male and female values showed a similar distribution and no age dependency.
Based on the distribution the cut-off value for elevated ccK18 has been set at > 200 U/L.
In case of suspected fibrosis or fatty liver disease:
ccK18 values  between 150 and 200 U/L suggest an indication for mild fibrosis (for example NAFL); ccK18 values > 200 U/L are a strong indication for severe significant liver damage (for example NASH).

Species

Human, non-human primates

Tests96
MethodELISA
Hook

No high dose “hook effect” occurs until 50000 U/L which is well above concentrations of K18Asp396-NE (M30)-reactive material observed in cell culture samples.

Intended use

The M30 neo-epitope (K18Asp396-NE) is a sensitive and integrative indicator specific for epithelial cell death involving caspase -3, -7 or -9 activation.

Quantitative measurement of the apoptotic cell death biomarker K18Asp396-NE (“M30 antigen”) released from dying human epithelial cell lines in vitro or in vivo. The cells or tissues should be of human epithelial origin (e.g. kidney, gut, colon, lung or liver) expressing K18.


* Note:
caspase-cleaved K18 = ccK18 previously Cytokeratin 18 (CK18/ccCK18)

Product informations

Back to overview