Liver

Product name

α-GST, Rat serum

Novel Liver Injury Biomarkers

Cat-Nr.TE1051
Range1.56 – 100 µg/l
Sensitivity0.2 µg/l
Incubation time2.5 hours
Sample volume100 μl
Sample type

Serum (1:50)
Cell Culture Supernatant (1:5 – 1:30 dependant on expected concentration)

Sample preparation

For serum sample analysis, blood samples should be allowed to clot at room temperature for 2 hours or 2 – 8 ºC overnight. The sample is then centrifuged (3000 rpm/10 minutes) and the serum collected. Serum samples should be assayed immediately or may be stored at 2 – 8 ºC for 4 days.
If necessary, the samples may be frozen at -20 ºC. Avoid repeated freezing and thawing. Do not store diluted samples. Samples can be stored at -20ºC for at least one month.

Cell Culture Supernatant:

A hepatocyte cell number of 4 x 105 cells /ml cell culture medium is recommended.

Reference values

Sprague-Dawley Rats 43 ± 56μg/l (mean ± 2SD)
Normal ranges may vary between different rat strains. Therefore, it is important for each laboratory to assess a normal range for the rat strain used in the study.

Species

Rat

Cross reaction

No significant cross-reactivity is observed with Yp or Yb1 isoforms.
Cross reactivity with human, canine and porcine αGST is undetectable.

Tests96
MethodELISA
Intended use

α-GST is a member of the GST superfamily of small cytosolic proteins primarily involved in cellular detoxification reactions. α-GST is approximately 51kDa and can comprise upto 2% of the cytosolic soluble protein content. α-GST is expressed in the cytosol of epithelial cells of the proximal tubule. As a result of its constitutive nature, α-GST is immediately released into the urine upon lysis of epithelial cells of the proximal tubule.
In the liver, α-GST is expressed at high concentrations in the cytosol of hepatocytes throughout the liver parenchyma and is rapidly released upon cellular lysis making it a sensitive biomarker of acute hepatic injury

In the event of liver injury, α-GST is released more rapidly than transaminases and has a shorter half-life in the circulation. Therefore, α-GST levels more accurately indicate the onset and resolution of hepatocyte injury than transaminases. α-GST has been proven to be a superior indicator of hepatocyte injury in hepatotoxicity, transplantation and ischemia-reperfusion injury.

Product informations

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