Liver

Product name

α-GST, Human serum & plasma

Novel Liver Injury Biomarkers

Cat-Nr.BIO91
Range2.5 - 80 μg/l
Sensitivity1.9 μg/l
Incubation time2 hours
Sample volume100 μl (Suggested initial dilution 1:5)
Sample type

Serum & Plasma

Sample preparation

Centrifuge within 3 hours from time of collection and transfer the sample from the original tube for storage. Samples can be stored at 20 – 25 °C for up to 48 hours, at 2 – 8 °C for up to one week or at -20 °C for >1 year. Repeated freeze thawing of samples should be avoided.

Reference values

0 - 12 μg/l

Species

Human

Cross reaction

No cross-reactivity was observed with μGST at 500 μg/l or π-GST at 500 μg/l

Tests96
MethodELISA
Note

Serum & Plasma:

α-GST is expressed in hepatocytes and its increase in blood is a sensitive indicator of acute hepatic injury. Increased αGST has been demonstrated to be indicative of hepatotoxicity, allograft rejection, viral hepatitis.

Intended use

α-GST is a member of the GST superfamily of small cytosolic proteins primarily involved in cellular detoxification reactions. α-GST is approximately 51kDa and can comprise upto 2 % of the cytosolic soluble protein content. As a result of its constitutive nature, α-GST is immediately released into the urine upon lysis of epithelial cells of the proximal tubule.

The α-GST EIA provides a method for the quantitative determination of alpha glutathione S-transferase
(α-GST) in human serum and plasma. The α-GST EIA can also be used for the determination of αGST in human urine, to assess kidney injury.

Product informations

Back to overview