Kidney disease

Product name

Collagen IV, Human urine

Kidney Injury Biomarker for progression of renal fibrosis 

Cat-Nr.TE1054
Range0.8 – 50 μg/l
Sensitivity0.8 μg/l
Incubation time25 hours
Sample volume50 μl
Sample type

Urine

Sample preparation

If left standing Collagen IV can precipitate out of urine resulting in falsely low reported results. For this reason we recommend the use of Collagen IV sample collection tubes mwhich ensure accurate reproducible measurement. After collection in this manner samples may be stored at 2-8 °C for one week or at -20 °C for nine months.
Care should be taken to avoid freeze thaw cycles.

Reference values

Reference values are dependent on the type of sample collection proceedure.

 

    Collagen IV (μg/g Creatinine) N
Early morning urine 30-39 years <4,0 122
Early morning urine > 40 years < 4,9 64
Spot urine < 21 years < 7,3 390

 

Species

Human

Cross reaction

Cross reactivity is less than 2 % with Collagen II and less than 0.5 % with other forms of collagen.

Tests96
MethodELISA
Note

Interference:

No significant interference has been observed in this assay with creatinine, hemolytic or icteric samples.

  • Creatinine: Less than 10 % interference up to 3 g/l in sample.
  • Haemolysis: Less than 10 % interference up to 4.8 g/l haemoglobin.
  • Icterus: Less than 10 % interference up to 0.2 g/l bilirubin.
Intended use

Collagen IV (Col IV) is a large extracellular protein and a major component of the mammalian extracellular matrix. It is also a key component of the glomerular basement membrane (BM) and tubular BM, and is considered to constitute their basic framework. Glomerular BM expansion is a hallmark of several chronic renal pathologies, with increased urinary Col IV being indicative of the extent of these morphological alterations.

This Coll IV EIA provides a method for the quantitative determination of Col IV in human urine. Col IV levels are elevated in a variety of renal pathologies and have been found to correlate with disease staging eg. diabetic and non-diabetic nephropathies, renal transplantation and acute rejection Increased Col IV has been demonstrated to correlate directly with the rate of renal functional decline in type 1 and type 2 diabetics with urinary Col IV levels being demonstrated to be a more sensitive marker of disease progression than urinary albumin levels.

Product informations

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