Kidney disease

Product name

π-GST, Human urine

Kidney Injury Biomarker for distal tubular damage

Cat-Nr.BIO85RUO
Range1.25 - 40 μg/l
Sensitivity1.3 μg/l
Incubation time2.5 h
Sample volume100 μl
Sample type

Urine (Suggested initial dilution of 1:2)

Sample preparation

If the sample seems to contain blood, it must be centrifuged prior to processing and testing (see IFU for full instructions). Once clear of haemolysis contamination, sample should be diluted with urine stabilizing buffer (USB). Sample: USB 4:1.
It is recommended that samples are assayed as soon as possible after collection.
After the addition of USB, samples can be stored at 20-25 °C for up to 48 hours, at 2-8 °C for up to one week, or at -20 °C for >1 year.
Repeated freeze-thaw cycles should be avoided.

Reference values

0 – 30 µg/l (n=132)

Species

Human

Cross reaction

No cross-reactivity was observed with μ-GST at 500 μg/l or α-GST
at 500 μg/l. 

Tests96
MethodELISA
Intended use

Pi Glutathione S-Transferase (π-GST) is a member of the GST superfamily of small cytosolic proteins primarily involved in cellular detoxification reactions. π-GST is approximately 47kDa and can comprise up to 2 % of the cytosolic soluble protein content. As a result of its constitutive nature, π-GST is immediately released into the urine upon lysis of epithelial cells of the distal tubule.

Intended use:

The π-GST EIA provides a method for the quantitative determination of π-GST in human urine.

π-GST is specifically expressed in epithelial cells of the distal tubules of the human kidney whereas α-GST expression is confined to cells of the proximal tubules. π-GST is readily released from epithelial cells upon cellular lysis making it a rapid and sensitive indicator of acute kidney injury (AKI) onset in cases of nephrotoxicity, environmental toxicity, cardiothoracic surgery and the intensive care setting. π-GST has also demonstrated clinical value in assessing transplant rejection and diabetes.
π-GST has been demonstrated to be an excellent predictor of severe acute kidney injury leading to dialysis requirement in cardiothoracic surgery. The combination of sensitivity and specificity makes urinary π-GST the biomarker of choice when accurate diagnosis of renal acute distal tubular injury is required.

Product informations

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