Apoptosis

Product name

M65 EpiDeath® ELISA (PEVIVA®)

M65 Apoptosis + Necrosis. Human intermediate filament protein keratin 18 (K18)*. Released from human epithelial cells.

Cat-Nr.10040
Range200 - 5000 U/l ( 1 U/l = 1.24 pM)
Sensitivity25 U/l
Incubation time4.5 hours
Sample volume25 µl
Sample type

Human serum or plasma (EDTA, Citrate, Heparin), Cell culture
supernatants from cells or tissues.

Sample preparation

Store the samples at 2–8 °C up to 4 hours. For longer periods, store samples at -20 °C or lower.
Avoid repeated freezing and thawing of specimens.

Reference values
≤ 128 U/l Normal
128 – 183 U/l Greyzone
> 183 U/l Elevated (if carcinoma is excluded)

 

Species

Human, monkey.

Specificity

The assay uses two monoclonal antibodies directed to epitopes in the
284 – 396 region of the K18 protein. Soluble full length K18 as well as K18 fragments and protein complexes that expose these epitopes will be detected by the assay.

Tests96
MethodELISA
Hook

No high dose “hook effect” occur until 200000 U/l which is well above concentrations of M65-reactive material observed in cell culture or serum/plasma samples.

Intended use

Quantitative measurement of total soluble keratin 18 (K18) released from dead cells (necrotic and apoptotic). The cells or tissues should be of human epithelial origin (e.g. kidney, gut, colon, lung or liver) expressing K18.

The M65 EpiDeath® ELISA can be combined with the M30 Apoptosense® ELISA (PEVIVA Prod. No. 10010) for determination of cell death mode (apoptosis versus necrosis). Death mode can be determined in vitro and in serum from cancer patients.


*Note:
caspase-cleaved K18 = ccK18 previously Cytokeratin 18 (CK18/ccCK18)

Product informations

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