ELISA Complement Activity Analysis

Product name

HUMAN MBL

Cat-Nr.TE323
Range0.41 to 100 ng/ml
Sensitivity0.41 ng/mL (Detection limit)
Incubation time4 hours
Sample volume100 ┬Ál (diluted 1:50-1:100)
Sample type

Precondition: Use polypropylene tubes
Serum, plasma (heparin, citrate, not EDTA)
Hemolyzed, hyperlipemic, heat-treated or contaminated samples
may give erroneous results.

Sample preparation

Store samples below -20°C, preferably at -70°C.
Use samples within 24 hours after thawing.
Avoid multiple freeze-thaw cycles

Reference values

10 to 5,000 ng/ml
12% of healthy Caucasian blood donors have MBL concentrations below 100 ng/ml.

Species

Human

Cross reaction

Cross-reactivity for other species or proteins/peptides has not been test

Tests96
MethodELISA
Note

Interferences: This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples.

Intended use

Mannose Binding Lectin (MBL) is a C-type lectin that is an important element in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species.It forms a complex with C1r/C1s like serine proteases designated MASP that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent anti-bacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. Low plasma concentrations have been associated with an inherited defect in opsonization. The MBL concentration is enhanced in infectious diseases.

Measuring of MBL is indicated in:

  • recurrent infections (especially in children),
  • primary/secondary immunodeficiencies,
  • artherosclerosis/coronary heart disease,
  • cystic fibrosis,
  • transplantation
  • autoimmune diseases (SLE/Rheumatoid arthritis)
Product informations

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