ELISA Complement Activity Analysis

Product name

Bb Plus MicroVue™ Quidel®

Bb fragments of Factor B of the Alternative Complement Pathway

Cat-Nr.A027
Range0.06 - 0.65 µg/ml
SensitivityLOD: 0.018 µg/ml; LLOQ: 0.033 µg/ml
Incubation time1,5 Stunden
Sample volume100 µl (after dilution 1:10 for plasma, 1:20 for serum)
Sample type

Serum and plasma

Sample preparation

Specimens should be tested immediately or stored at 4 ºC or on ice until assayed. This should not exceed four hours.
For long-term storage, freeze at -70ºC within two hours after collection.

Reference values

in Plasma 0.49 - 1.42 µg/ml (+2 SD)
in Serum  0.80 - 6.26 µg/ml (+2 SD)

Species

Human, cynomolgus macaque, baboon, rhesus macaque

Specificity

Monoclonal mouse-antibody, specifically binds Bb.

Tests96 Tests
MethodELISA
Intended use

The Bb fragment enzyme immunoassay measures the amount of the complement fragment of Bb, an activation fragment of Factor B in human plasma or serum, as well as in other biological fluids, experimental samples or mixtures. This measurement allows a quantitative assessment of the extent of activation of the alternative pathway of complement in the test sample. Measurement of alternate pathway activation aids in the diagnosis of several kidney diseases (e.g. chronic Glomerulonephritis, lupus nephritis), as well as several skin diseases (e.g. dermititis herpetiformis and pemphigus vulgaris). Other diseases in which activation of the alternat pathway of complement has been observed include rheumatoid arthritis, sickle cell anemia,
and gram-negative bacterial infections.

Product informations

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