Calcium metabolism

Product name

PTH 1-84 Intact, Rat

Cat-Nr.60-2500
Range25 – 840 pg/ml (can be extended to 2400 pg/ml)
Sensitivity1.6 pg/ml
Incubation time3.5 hours
Sample volume25 µl
Sample type

Serum, EDTA plasma, cell culture

Sample preparation

Samples should be tested immediately or stored frozen at -20°C or below.

Species

Rat, (mouse), bovine

Cross reaction

Human PTH 1-84: 2.3%.

Standards

Synthetic rat PTH 1-84

Specificity

Detects intact PTH 1-84. PTH 1-34 and 39-84 are not detected. Cross-reactivity with human PTH 1-84 2.3%.

Tests96 Tests
MethodELISA
Intended use

PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized in the parathyroid gland as a pre-proparathyroid hormone, a larger molecular precursor consisting of 115 amino acids. Following sequential intracellular cleavage of a 25-amino acid sequence, pre-proparathyroid hormone is converted to an intermediate, a 90-amino acid polypeptide, proparathyroid hormone. By additional proteolytic modification, proparathyroid hormone is then converted to parathyroid hormone, an
84 amino acid polypeptide. In healthy individuals, regulation of parathyroid hormone secretion normally occurs via a negative feedback action of serum calcium on the parathyroid glands. Intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes. PTH undergoes proteolysis in the parathyroid glands, but mostly peripherally, particularly in the liver but also in the kidneys and bone, to give N-terminal fragments and longer lived C-terminal and Mid-region fragments. Intact PTH assays are important for the differentiation of primary hyperparathyroidism from other (non-parathyroid-mediated) forms of hypercalcemia, such as cancer, sarcoidosis and thyrotoxicosis.

Product informations

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