Calcium metabolism

Product name

PTH 1-84 Intact, Mouse

Cat-Nr.60-2305
Range25 – 840 pg/ml (can be extended to 2700 pg/ml)
Sensitivity4 pg/ml
Incubation time3.5 hours
Sample volume20 µl
Sample type

EDTA plasma, cell culture

Sample preparation

Samples should be tested immediately or stored frozen at -20°C or below.

Species

Mouse, rat

Cross reaction

No cross-reaction with PTH (1-34), mid-region and PTH (39-84)

Standards

Synthetic mouse intact PTH 1-84. Mouse PTH values are indicated as rat PTH equivalents.

Specificity

Antibodies directed against mouse and 
rat PTH (39-84 and 1-34) detect intact PTH.

Tests96 Tests
MethodELISA
Note

 

Mouse species   CD-1 BALB C57BL Swiss Webster
  N 20 20 20 20
Male Mean 152 192 53 92
  SD 69 45 20 15
  Min 76 137 27 67
  Max 365 273 108 123
Range Mean -1SD 83 147 33 77
  Mean +1SD 221 236 73 107
  N 20 20 20 20
Female Mean 105 160 117 102
  SD 29 37 14 22
  Min 71 120 91 65
  Max 182 233 145 139
Range Mean -1SD 76 122 103 81
  Mean +1SD 133 197 131 124
Intended use

PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized in the parathyroid gland as a preproparathyroid hormone, a larger molecular precursor consisting of 115 amino acids. Following sequential intracellular cleavage of a 25-amino acid sequence, pre-proparathyroid hormone is converted to an intermediate, a 90-amino acid polypeptide, proparathyroid hormone. By additional proteolytic modification, proparathyroid hormone is then converted to parathyroid hormone, an 84 amino acid polypeptide. In healthy individuals, regulation of parathyroid hormone secretion normally occurs via a negative feedback action of serum calcium on the parathyroid glands. Intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes. PTH undergoes proteolysis in the parathyroid glands, but mostly peripherally, particularly in the liver but also in the kidneys and bone, to give N-terminal fragments and longer lived C-terminal and Mid-region fragments. Intact PTH assays are important for the differentiation of primary hyperparathyroidism from other (non-parathyroid-mediated) forms of hypercalcemia, such as cancer, sarcoidosis and thyrotoxicosis.

Product informations

Back to overview