Calcium metabolism

Product name

PTH, 1-84, Horse, Bovine, Dog, Human

Cat-Nr.60-3100
Range10 - 1000 pg/ml
Sensitivity4 pg/ml
Incubation time3.5 hours
Sample volume25 ┬Ál
Sample type

Serum, EDTA plasma, cell culture

Sample preparation

Samples should be tested immediately or stored frozen at -20°C or below.

Reference values

20 - 120 pg/ml, mean 56 pg/ml

Species

Horse, cattle, pig, human

Cross reaction

100% with PTH 7-84

Standards

Synthetic human PTH 1-84

Specificity

Antibody agains human PTH 39-84 and PTH 1-34, measurement of intact PTH.

Tests96 Tests
MethodELISA
Intended use

PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized in the parathyroid gland as a preproparathyroid hormone, a larger molecular precursor consisting of 115 amino acids. Following sequential intracellular cleavage of a 25-amino acid sequence, pre-proparathyroid hormone is converted to an intermediate, a 90-amino acid polypeptide, proparathyroid hormone. By additional proteolytic modification, proparathyroid hormone is then converted to parathyroid hormone, an 84 amino acid polypeptide. In healthy individuals, regulation of parathyroid hormone secretion normally occurs via a negative feedback action of serum calcium on the parathyroid glands. Intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes. PTH undergoes proteolysis in the parathyroid glands, but mostly peripherally, particularly in the liver but also in the kidneys and bone, to give N-terminal fragments and longer lived C-terminal and Mid-region fragments. Intact PTH assays are important for the differentiation of primary hyperparathyroidism from other (non-parathyroid-mediated) forms of hypercalcemia, such as cancer, sarcoidosis and thyrotoxicosis.

Product informations

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