Calcium metabolism

Product name

PTH 1-34 Human, high sensitive

Cat-Nr.60-3900
Range10 - 330 pg/ml
Sensitivity0.9 pg/ml
Incubation time3.5 hours
CalibrationWHO NIBSC 04/200
Sample volume150 ┬Ál
Sample type

EDTA plasma, cell culture

Sample preparation

Samples should be tested immediately or stored frozen at -20° or below.

Species

Human

Cross reaction

 

  Molar (%)
Human PTH (1-34) 100.0
Human PTH (1-84) 2.8
Human PTH (7-84) <0.0004
Ratte PTH (1-34) 43.6
Ratte PTH (1-84) 3.0

 

Standards

Synthetic PTH 1-34. WHO No. 04/200 Standardization.

Tests96 Tests
MethodELISA
Intended use

Over the years, studies involving the intermittent dosing of human PTH (1-34) in animals and humans have demonstrated an anabolic effect on bone. This effect was positive for bone mass, size, structure, and strength, demonstrating the antifracture efficacy of the treatment. Recently the FDA has approved the recombinant form of human PTH (1-34) - rhPTH (1-34) or teriparatide, for treatment of many forms of osteoporosis. The measurement of circulating levels of this peptide/drug may be useful in assessing its pharmacological properties and for appropriate dosing and schedule optimization. Additionally, this assay may be useful in screening samples for both endogenous levels of circulating hPTH (1-34) and elevated levels of this peptide as it may interfere to varying degrees with 2-site immunometric assays used for measuring intact PTH (1-84).

Obtaining precise measurements of human PTH (1-34) are complicated by antibody cross-reactivity to endogenous PTH (1-84), requiring either a pre-assay immunoextraction of PTH (1-84) or assaying for both PTH (1-34) and intact PTH (1-84) to account for this cross-reactivity.

This High Sensitivity (HS) Human PTH (1-34) ELISA Kit uses two antibodies that have been prepared for optimal recognition of human PTH (1-34) while minimizing binding to human PTH (1-84).

Product informations

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