Animal Assay

Product name

Equine Haptoglobin

Cat-Nr.TE1032
Range0 – 10 mg/ml, 0- 1000 ng/ml (range after dilution)
Sensitivity0.026 mg/ml, 2.6 ng/mL (LLOQ after dilution)
Incubation time2 hours
Sample volume20 μl (dilute 1:10000)
Sample type

Serum, plasma and cell culture

Sample preparation

Centrifuge collected blood samples within 4 hours. Samples are stable for 72 hours at 2-8 °C or room temperature, long term storage at -20°C, or at -70°C.
Maximum 3 freeze- and thaw cycles.

Reference values

Serum of healthy horses < 1mg/ml

Species

Equine

Tests96
MethodELISA
Note

Interference:

No interference with hemoglobin in hemolytic samples.

Background:

Haptoglobin, an acute phase protein, is part of immune system-mediated defence mechanisms found in the blood various animal species.
Under normal conditions, haptoglobin is either absent from the blood or present at very low levels.
However, haptoglobin can increase significantly in response to acute infection, inflammation or trauma.
Several functional properties of haptoglobin have been described.
The major biological function of haptoglobin is to bind free hemoglobin in an equimolar ratio with very high affinity to prevent hemoglobin-mediated renal parenchymal injury and loss of iron following intravascular hemolysis. The complex of haptoglobin with hemoglobin is metabolized in the hepatic reticuloendothelial system. Biosynthesis of haptoglobin occurs not only in the liver, but also in adipose tissue and lung, providing antioxidant and antimicrobial activity.

Intended use

Several studies in horses with respiratory and orthopedic disorders or after surgical interventions have shown that Haptoglobin is a general, not disease specific parameter for inflammatory processes and therefore an alternative to serum amyloid A or fibrinogen.

In equine medicine haptoglobin as acute phase protein is helpful in diagnostic and therapy of various diseases.

  • Rapid and easy detection of inflammatory processes
  • Indicator for respiratory and orthopedic disorders
  • Monitoring of therapy (Immune-mediated inflammatory processes)
  • Screening before and follow-up after surgery
  • Helpful as an aid in the diagnosis of infectious diseases and cancer
  • Screening of health status,
Product informations

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