ELISA Complement Activity Analysis

Product name

iC3b MicroVue™ Quidel®

Quantification of the iC3b fragment of C3 Protein

Cat-Nr.A006
Range0.2 - 2.0 µg/ml
Incubation time1.5 hours
Sample volume100 µl (after dilution 1:25 – 1:200)
Sample type

Serum and EDTA-plasma

Sample preparation

The proper collection and storage of specimens is essential, since C3 is highly susceptible to proteolysis and hydrolysis. Serum or EDTA plasma specimens should be collected aseptically using standard techniques. They should be tested immediately or stored at 4 ºC or on ice until assayed. This should not exceed 4 hours. For long-term storage, freeze at -70 ºC within 2 hours after collection.

Species

Human

Specificity

Anti-iC3b monoclonal anti body specifically binds iC3B and not to C3.

Tests96 Tests
MethodELISA
Intended use

The iC3b enzyme immunoassay measures the amount of the iC3b fragment in human plasma or serum,  as well as in other biological fluids, experimental samples or mixtures.
The levels of iC3b can be significantly elevated in the serum and plasma of some patients with complex-associated diseases such as rheumatoid arthritis and systemic lupus erythmatosus. iC3b levels may also be elevated in body fluids from other patients with infections, burns, myocardial infarctions, glomerulonephritis, and acute respiratory distress syndrome.

Product informations

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