ELISA Complement Activity Analysis

Product name

SC5b-9 Plus MicroVue™ Quidel®

Quantification of the SC5b-9 complex

Cat-Nr.A029
Range10 - 170 ng/ml
SensitivityLOD: 3.7 ng/ml; LLOQ: 8.8 ng/ml
Incubation time2 hours
Sample volume10 µl (dilute 1:40 for serum); 50 µl (dilute 1:10 for plasma)
Sample type

Serum, EDTA plasma, spinal fluid or other biological fluids

Sample preparation

The proper collection and storage of specimens is essential since SC5b-9 may be generated in improperly handled specimens. Serum or EDTA plasma specimens should be collected aseptically using standard techniques.
They should be tested immediately or stored at 4 ºC or on ice until assayed. This should not exceed four hours. For longer-term storage freeze at -70 ºC. Plasma concentrations better reflect in vivo concentrations in comparison to serum concentrations.

Reference values

Serum              334 - 1672 ng/ml
EDTA Plasma     127 - 303 ng/ml

Species

Human, cynomolgus macaque, baboon, rhesus macaque

Specificity

Monoclonal mouse-antibody, specifically binds SC5b-9.

Tests96 Tests
MethodELISA
Intended use

The SC5b-9 enzyme immunoassay measures the amount of SC5b-9 present in human plasma, serum and other biological or experimental samples.
The Terminal Complement Complex (TCC, SC5b-9) is generated by the assembly of C5 through C9 as a consequence of activation of the complement system by either the classical, lectin or alternative pathway. The membrane attack complex (MAC), a form of TCC, is a stable complex that mediates the irreversible target cell membrane damage associated with complement activation.

Product informations

Back to overview