ELISA Complement Activity Analysis

Product name

MBL-C Mouse

Cat-Nr.TE209
Range3.1 to 200 ng/ml
Sensitivity3.1 ng/mL (Detection limit)
Incubation time3.5 hours
Sample volume100 ┬Ál/well (diluted samples)
Sample type

Precondition: Use polypropylene tubes.
Serum, EDTA plasma, cell culture supernatant
Hemolyzed, hyperlipemic, heat-treated or contaminated samples
may give erroneous results.

Sample preparation

Store samples below -20°C, preferably at -70°C.
Use samples within 24 hours after thawing.
Avoid multiple freeze-thaw cycles

Reference values

16 to 118 µg/ml (plasma of healthy mice)

Species

Mouse

Cross reaction

Cross-reactivity for other species or proteins/peptides has not been tested.

Tests96
MethodELISA
Note

Interferences: This assay is designed to eliminate interference by soluble receptors, binding proteins, and other factors present in biological samples.

Intended use

Mannose Binding Lectin (MBL) is a C-type lectin that is an important element in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species.It forms a complex with C1r/C1s like serine proteases designated MASP that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent anti-bacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. Only one form of human MBL has been characterized, while two forms are found in rhesus monkeys, rabbits, rats and mice. The murine forms are known as MBL-A and MBL-C. The MBL-C concentrations in serum are about 6-fold higher compared to that of MBL-A. MBL-A, but not MBL-C was found to be an acute phase protein in casein and LPS-injection models. MBL-C exists in higher oligomeric forms than MBL-A. In infectious diseases the MBL-C concentration was not found to increase significantly.

Product informations

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