ELISA Complement Activity Analysis

Product name

C4d MicroVue™ Quidel®

Quantification of C4d-containing fragments of activated C4 of the Classical Complement Pathway

Cat-Nr.A008
Range0.025 - 0.25 µg/ml
SensitivityLOD: 0.01 µg/ml; LLOQ: 0.022 µg/ml
Incubation time1.5 hours
Sample volume10 µl (dilute 1:70 for normal Samples)
Sample type

Serum, EDTA plasma or other biological fluids

Sample preparation

The proper collection and storage of specimens is essential, since C4d is highly susceptible to proteolysis. Serum or EDTA plasma specimens should be collected aseptically using standard techniques. They should be tested immediately or stored at 4 ºC or on ice until assayed. This should not exceed four hours. For long-term storage freeze at -70 ºC within two hours after collection.

Reference values

EDTA Plasma    0.7 µg – 6.3 µg/ml (+2SD)
Serum               1.2 µg – 8.0 µg/ml (+2SD)

Species

Human, cynomolgus macaque, baboon, rhesus macaque

Specificity

Monoclonal mouse-antibody, specifically binds C4d.

Tests96 Tests
MethodELISA
Intended use

The C4d fragment enzyme immunoassay measures the amount of the C4d-containing activation fragments of C4 (C4b, iC4b, and C4d) present in human serum, EDTA plasma and other biological or
experimental samples.
The levels of C4d, when normalized for the presence of endogenous C4, can be significantly elevated is plasma specimens obtained from some patients with rheumatoid arthritis, hereditary angioedema, systemic lupus erythematosis and other illnesses. Cd4 may also be elevated in body fluids and plasma samples obtained from patients in which classical complement pathway activation is known to occur, e.g. from patients with a fariety of humoral autoimmune diseases, septicemia, thermal injury, multiple organ trauma, myocardial infarction, hereditary angioedema, glomerulonephritis and acute respiratory distress syndrome.

Product informations

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