Oxidative Stress & Cardiovascular Disease

N-terminal fragment of atrial natriuretic propeptide 1-98

EDTA- or Heparin plasma, serum, urine, cell culture supernatant

Centrifuge freshly collected blood within one hour, test immediately or store at -20°C, for longer storage at -70°C. The stability of NT-proANP 1-98 in serum compared to plasma is lower.
4 freeze-thaw cycles are possible for plasma. 2 cycles for serum.
Urine and cell culture supernatant can be measured directly.

Plasma median: 1,45 nmol/l

Human, rat, mouse, porcine

proANP (1-30): <1%, proANP (31-67): <1%, proANP (79-98): <1%,
alpha ANP (99-126): <1%, proBNP (8-29): <1%, proBNP (32-57): <1%,
proCNP (1-19): <1%, proCNP (30-50): <1%, proCNP (51-97): <1%

Atrial natriuretic peptide is synthetisized in atrial myocytes and is stored in secretory granules as a 126 amino acid prohormone. The most important stimulus for the release of the hormone into circulation is stretch of the myocyte fibres. On release the prohormone is split into equimolar amounts of the highly biologically active proANP (99-126), also known as alpha-ANP, and the N-terminal part proANP (1-98). Alpha-ANP is rapidly cleared from the circulation with a half-life of 3-4 minutes. proANP (1-98) has a much longer half-life (60-120 min) which leads to significantly higher concentrations in blood compared to alpha-ANP. Thus, circulation levels of proANP (1-98) are less sensitive to the pulsatile secretion of ANP and may better reflect chronic levels of ANP secretion than the rapid fluctuating levels of alpha-ANP. proANP is discussed as valuable marker for e.g. sepsis or risk stratification in heart failure.

Clinical significance:
• research studies on heart failure (LVD, CHF etc)
• research studies on heart transplanted patients
• drug studies on heart transplanted patients
• risk assessment in heart failure
• risk assessment in MI patients with normal NT-proBNP levels
• monitoring of cardiac resynchronization therapy