| Anwendung |
C4 is one of the proteins unique to the Classical Complement Pathway. The 200 kD C4 protein is made up of three peptide chains linked by disulfide bonds and contains a thioesterbond. When the classical pathway is activated, C1s cleaves C4 to C4a (a weak anaphylatoxin) and C4b. C4b can then bind covalently to a target surface or complex. It can then function as an opsonin or participate as part classical pathway convertase. C4 has been shown to exist as two separate "complotypes " C4A and C4B. These have been shown to have different biological properties.
Specifications:
Concentration: 1.0 mg/ml
Volume/Vial: 250 µl/vial
Storage: at or below -70°C
Purity: > 95%
Buffer: PBS
Functional Testing:
All Quidel complement components are tested for functional activity in a standard lytic or applicable functional assay and for biochemical purity by SDS- polyacrylamide gel electrophoresis. Please refer to the Certificate of Analysis for lot specific information on functional titer.
Applications:
Quidel's purified human complement components are useful in a wide variety of immunochemical and research applications.
References:
- Schifferli JA, Paccaud J., Two Isotypes of human C4, C4A and C4B, have different structure and function, Complement and Inflammation 1989;6:19-26.
- Nicholson-Weller, Butko, P, Wessels, M., Role of Complement Component C1q in IgG Independent Opsonophagocytosis of Group B Streptococcus, J. Immunol 163:2761-2768, 1999.
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