Growth metabolism

Productname IGFBP-2 Mouse / Rat

Insulin like Growth Factor Binding Protein-2 for mouse and rat

Cat-No. E08

Range 31 – 2000 pg/ml (after dilution 1:100)

Sensitivity 0.01 ng/ml

Incubation time 3 hours RT

Sample volume 10 µl (dilute 1:100)

Sample type

Serum

Sample preparation

Samples have to be chilled as soon as possible after collection. Long-term storage should be carried out at –20°C or below. Avoid repeated freezing/thawing of specimens.

Reference values

	range (ng/ml)	median (ng/ml )
mouse	59.7 – 105.7	81.0
rat	10.7 – 38.1	24.2

Species

mouse, rat

Tests 96 Tests

Method ELISA

Product informations - Cross-reaction all species (pdf-File 71 kb)

Intended use

IGFBP-2 is an unglycosylated polypeptide of 31.3 kDa, which forms binary IGF-complexes and shows no circadian rhythm in the circulation.

The serum concentration of IGFBP-2 increases in fasting, after major surgery and after trauma, but the increasing of the concentration is most intensive in malignant diseases. The correlation of the IGFBP-2 level to the degree of progression is a striking feature in various tumor types as is the normalization of the IGFBP-serum levels after remission. During the GH-therapy, e.g. in short stature and in GH-abuse (doping) the IGFBP-2 level decreases. In Trisomy 18 IGFBP-2 in maternal serum is decreased and IGFBP-1 is increased; therefore the ratio IGFBP-2/IGFBP-1 is a marker for this chromosome abnormality.

Especially in tumor biology the mouse and rat systems enable investigation of the systemic relevance of IGFBP-2. IGFBP-2 influences tumor cells as it induces catalase activity in adrenocortical cells. Furthermore IGFBP-2 interacts with tumor cells via its RGD-amino acid sequence and seems to stimulate cell invasion of glioma cells.

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