ELISA Complement Activity Analysis

Quantification of the Complement Ba fragment

EDTA Plasma, serum, urine

Serum / Plasma:
The Ba fragment of Factor B is susceptible to proteolysis. For optimal plasma results, K2 EDTA should be used. Collect blood sample and centrifuge immediately at 2-8°C. Assay immediately, do not store longer than 2 hours at 2-8°C. For longer storage -70°C.
Urine:
Collect preservative-free first Morning void (FMV) or second morning void (SMV) before 10:00 am. Store sample refrigerated (2-8°C) for less than
1 day, or freeze the sample at -70°C for longer storage.
Maximum 5 freeze and thaw cycles.

Human, African green monkey, cynomolgus monkey, rhesus monkey,
canine

Monoclonal mouse-antibody, specifically to capture the Ba fragment.

By quantifying the amount of Ba, the extent of alternative pathway activation at the time of sample collection can be determined.

Activation of the alternative pathway has been associated with a variety of disease states including SLE, chronic glomerulonephritis, rheumatoid arthritis, sickle cell anemia and gram negative bacterial infections.

The activation of the alternative complement pathway can be triggered by a variety of substances including microbial polysaccharides or lipids, gram-negative bacterial lipopolysaccharides, surface determinants present on some viruses, parasites, virally infected mammalian cells, and cancer cells. In autoimmune
diseases, the alternative complement pathway may contribute directly to tissue damage. Alternative complement pathway activation may also be an indicator of hemo-incompatibility of biomaterials.

Application

• kidney diseases
  chronic glomerulonephritis
  lupus nephritis
• skin diseases
  dermatitis herpetiformis
  pemphigus vulgaris
• age-related macular degeneration
• fetal loss in at risk pregnancy
• rheumatoid arthritis
• sickle cell anemia