Calcium metabolism Product details

Samples should be tested immediately or stored frozen at -20°C or below.

Human, rat, mouse, porcine, bovine, (dog), rhesus macaque, Cynomolgus macaque

• Human PTH (1-34): 100%
• human PTH (1-84): 30%
• rat PTH (1-84): 47%

Antibody directed against rat PTH 1-34, detects intact PTH and PTH 1-34. The capture antibody is binding to epitopes in the 13–34 region of the molecule, whereas the detection antibody recognize epitopes within the 1-12 region.

PTH (Parathyroid hormone, Parathormone, Parathyrin) is biosynthesized in the parathyroid gland as a pre-proparathyroid hormone, a larger molecular precursor consisting of 115 amino acids. Following sequential intracellular cleavage of a 25-amino acid sequence, pre-proparathyroid hormone is converted to an intermediate, a 90-amino acid polypeptide, proparathyroid hormone. By additional proteolytic modification, proparathyroid hormone is then converted to parathyroid hormone, an
84 amino acid polypeptide. In healthy individuals, regulation of parathyroid hormone secretion normally occurs via a negative feedback action of serum calcium on the parathyroid glands. Intact PTH is biologically active and clears very rapidly from the circulation with a half-life of less than four minutes. PTH undergoes proteolysis in the parathyroid glands, but mostly peripherally, particularly in the liver but also in the kidneys and bone, to give N-terminal fragments and longer lived C-terminal and Mid-region fragments. Intact PTH assays are important for the differentiation of primary hyperparathyroidism from other (non-parathyroid-mediated) forms of hypercalcemia, such as cancer, sarcoidosis and thyrotoxicosis.