Bone metabolism Product details

Desoxypyridinolin-Crosslinks, Free and bound DPD

Serum, urine, cell culture

SERUM: Keep specimens refrigerated at 2-8°C for a maximum of 4 days or frozen at -20°C or below for longer storage. Before use, samples have to be hydrolyzed overnight. Protect from exposure to light.
URINE: Use preservative-free first or second morning urine. In case of longitudinal studies, urine should be collected at the same time every day. Store samples refrigerated at 2-8°C for a maximum of 7 days. For longer storage, freeze specimens at -20°C or below. Before use, samples have to be hydrolyzed overnight. Protect from exposure to light. URINE: Use preservative-free first or second morning urine. In case of longitudinal studies, urine should be collected at the same time every day. Store samples refrigerated at 2-8°C for a maximum of 7 days. Before use, samples have to be hydrolyzed overnight. Protect from exposure to light.

SERUM:

• Premenopausal women (25 – 44 years): 3.43 nmol/l, (S.D. 0.64 nmol/l)
• Men (25 – 55 years): 3.25 nmol/l, (S.D. 0.66 nmol/l)

URIN: 19 – 325 nmol/l

Weitz S, Benham P, Leung S (1999) Total deoxypyridinoline in serum and urine as measured by a novel adaptation of the Pyrilinks-D enzyme immunoassay. J Bone Miner Res 14 Suppl 1, 371 [Abstract].

Human, baboon, bovine, dog, guinea pig, horse, rhesus macaque, mouse, rat , rabbit, squirrel, cat, cynomolgus macaque, porcine, sheep, goat

Metra® Total DPD provides a quantitative measurement of the excretion of deoxypyridinoline (DPD) Crosslinks as an indicator of bone resorption. Elevated levels of urinary DPD indicate elevated bone resorption in individuals. Measurement of DPD is intended for use as an aid in monitoring bone resorption changes in postmenopausal women receiving hormonal or bisphosphonate anti resorptive therapies and in individuals diagnosed with osteoporosis. Approximately 45% of DPD is free, the remaining fraction is bound to oligopeptides ranging from small linear peptides to very large cross linked structures. Free and total DPD appear in healthy individuals just as in those with metabolic bone diseases.