Bone metabolism Product details

4936      Helical Peptide Control (Urine)     Set of 4 x 0.5 ml (4 levels)
Average: 23, 81, 240, 850 µg/l

Urine, cell culture

Collect preservative-free urine before 10 a.m. Keep specimen refrigerated at 2-8°C for storage up to 7 days. For long-term storage freeze sample at -20°C or below.

Adults (25 - 34 years), average 50.7µg/mmol Cr 
                                     (S.D. = 23.8 µg/mmol Cr).

Reference values from children are available.

Human, rat, rabbit, guinea pig, cat, dog, porcine, goat, sheep, bovine, horse, monkey, baboon, mouse, cynomolgus macaque

90% cross-reactivity with alpha1-(III) homologous peptides, no cross-reaction with alpha1-(II)- or alpha2-(I)homologous peptides.

The organic matrix of bone contains approximately 90% of type-I collagen, a helical, hetero-trimeric molecule comprising two α1 chains and one α2 chain. During bone resorption the collagen molecule is degraded and peptides of various molecular weights are released into the circulation, that are further degraded and/or released into the urine. A peptide consisting residues 620-633 derived from the helical region of the α1 chain was isolated from the urine of a patient with Paget’s disease. Thereafter, a synthetic peptide α1(I) 620-633 has been used as immunogen for monoclonal antibody production. These antibodies were used to develope a competitive immunoassay for the measurement of the Helical Peptide in human urine for bone metabolism studies. Increased levels of Helical Peptide have been found in urine of postmenopausal women with osteoporosis and urine of patients with Paget's disease. Both diseases are characterized by an enhanced degradation of bone collagen. Helical Peptide concentration decreased during estrogen or bisphosphonate therapies, indicating the anti-resorptive effect of these therapies.