Bone metabolism Product details

Productname	Dickkopf-1(DKK-1)
Cat-No.	BI-20412
Range	~ 3 - 50 pmol/l (1 pmol/l = 28.68 pg/ml)
Sensitivity	0.38 pmol/l
Incubation time	24 hours
Sample volume	50 µl (dilute 1:4)
Sample type	Serum, cell culture
Sample preparation	Samples store at -20°C. Avoid repeated freeze/thaw cycles. Lipemic or hemolyzed samples, as well as plasma should not be used. Before use, thaw samples at low temperature and mix them thoroughly.
Reference values	Serum samples of blood donors had a mean DKK-1 level of 52.6 pmol/l. Median 45.7 pmol/l, SD 20 pmol/l
Tests	96
Method	ELISA
Product informations	- Kit Instructions (pdf-File 323 kb) - Cross-reaction all species (pdf-File 71 kb) - Bone & Cartilage Metabolism (pdf-File 2480 kb)
Intended use	Dickkopf-1(DKK-1) is a 28,672 Da secreted protein that acts as soluble inhibitor of the WNT signalling pathway. This pathway contains lipid–modified glycoproteins that activate cell surface receptor-mediated signal transduction to regulate cell activities like: cell fate, proliferation, migration, polarity and gene expression. DKK-1 regulates different developmental processes and is also involved in the regulation of bone metabolism as it inhibits the differentiation of osteoblast. DKK-1 is also secreted by different tumours activity. Possible Indications • Osteoarthritis • Osteoporosis • Colon Carcinomas • Multiple Myelomas • Bone Metastases