| Productname |
 M65 EpiDeath® ELISA (PEVIVA®) |
|
M65 Apoptosis + Necrosis. Human intermediate filament protein keratin 18 (K18)*. Released from human epithelial cells. |
| Cat-No. |
10040 |
| Range |
200 - 5000 U/l ( 1 U/l = 1.24 pM) |
| Sensitivity |
25 U/l |
| Incubation time |
4.5 hours |
| Sample volume |
25 µl |
| Sample type |
Human serum or plasma (EDTA, Citrate, Heparin), Cell culture
supernatants from cells or tissues. |
| Sample preparation |
Store the samples at 2–8 °C up to 4 hours. For longer periods, store samples at -20 °C or lower.
Avoid repeated freezing and thawing of specimens. |
| Reference values |
| ≤ 128 U/l |
Normal |
| 128 – 183 U/l |
Greyzone |
| > 183 U/l |
Elevated (if carcinoma is excluded) |
|
| Species |
Human, monkey. |
| Specificity |
The assay uses two monoclonal antibodies directed to epitopes in the
284 – 396 region of the K18 protein. Soluble full length K18 as well as K18 fragments and protein complexes that expose these epitopes will be detected by the assay. |
| Tests |
96 |
| Method |
ELISA |
| Product informations |
- Kit Instructions (pdf-File 1,716 kb)
|
| Intended use |
Quantitative measurement of total soluble keratin 18 (K18) released from dead cells (necrotic and apoptotic). The cells or tissues should be of human epithelial origin (e.g. kidney, gut, colon, lung or liver) expressing K18.
The M65 EpiDeath® ELISA can be combined with the M30 Apoptosense® ELISA (PEVIVA Prod. No. 10010) for determination of cell death mode (apoptosis versus necrosis). Death mode can be determined in vitro and in serum from cancer patients.
*Note:
caspase-cleaved K18 = ccK18 previously Cytokeratin 18 (CK18/ccCK18)
|
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