| Productname |
M30 CytoDeath™ (PEVIVA®) |
|
Soluble caspase-cleaved fragments of the intermediate filament protein cytokeratin-18 (K18)* containing the M30 neo-epitope (K18-Asp396-NE).
Released from human, monkey or bovine epithelial cells in cell culture |
| Cat-No. |
10900 |
| Range |
250 - 3000 U/l (1 U/l = 1.24 pM) |
| Sensitivity |
60 U/l |
| Incubation time |
4.5 hours |
| Sample volume |
25 µl |
| Sample type |
Cell lysates or culture supernatants. |
| Sample preparation |
Fresh samples are stable for up to two days at 2 – 8 °C, for at least
9 months at -20 °C; and for at least two years when stored at -80 °C.
Avoid repeated freezing and thawing of specimens. |
| Species |
Cell lines from human, monkey and bovine. |
| Tests |
96 |
| Method |
ELISA |
| Product informations |
- Kit Instructions (pdf-File 435 kb)
|
| Intended use |
Quantitative measurement of the apoptotic cell death biomarker K18-Asp396-NE in cell culture experiments. Can be used for cell lysates and/or culture supernatants. The assay only detects apoptosis in cells of epithelial origin that express K18. Cells should be of human, monkey or bovine origin.
To be used to determine accumulation of caspase-cleaved K18 (ccK18) in cell cultures, providing an integrative measure of apoptosis. The K18-Asp396 neo-epitope is formed by caspase-3, -7 or -9 activation.
*Note:
caspase-cleaved K18 = ccK18 previously Cytokeratin 18 (CK18/ccCK18) |
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